Ankle stability in simulated lateral ankle ligament injuries
Related Experiment Video
Updated: Jun 12, 2026
A Mouse Model of Ankle-Subtalar Complex Joint Instability
Published on: October 28, 2022
Tadashi Fujii1, Harold B Kitaoka, Kota Watanabe
1Orthopaedic Biomechanics Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
Background:
We performed a laboratory-based study of the ankle-hindfoot complex in normal cadaveric ankles with simulated lateral ankle ligament injuries, utilizing an ankle stability testing device. The ankle stability testing device distinguished between intact, injury to anterior talofibular ligament (ATFL) and combined injury to ATFL and calcaneofibular ligament (CFL).
Materials And Methods:
Ankles were tested in an ankle stability testing device, with a specified inversion torque applied through the range of sagittal plane motion, then internal rotation torque applied through the entire range of sagittal plane motion. Three-dimensional motion of the calcaneus and talus in relation to the tibia were determined with a magnetic tracking system. The specimens were tested in the intact condition, then after sectioning the ATFL, then the CFL.
Results:
Upon sectioning the ATFL, there was a significant increase in the internal rotation motion at the tibiotalar joint. Subsequent sectioning of the CFL did not significantly increase ankle-hindfoot internal rotation, but it did significantly increase the motion in inversion testing. This increase occurred primarily at the tibiotalar level.
Conclusion:
The isolated ATFL injury was clearly distinguished from the intact condition by increased talar-tibial internal rotation, but not inversion. The more severe combined ATFL/CFL injury was distinguished from the ATFL injury condition with inversion torque.
Clinical Relevance:
Inversion torque may disclose combined ATFL/CFL injury, and internal rotation torque may be the key to distinguish isolated ATFL and combined ATFL/CFL injuries.
