Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 12, 2026

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
09:57

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

Mucin-lectin interactions assessed by flow cytometry.

Faye Jeffers1, Christine Fuell, Louise E Tailford

  • 1Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.

Carbohydrate Research
|June 19, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploratory analyses of cervicovaginal mucus O-glycan composition and microbiota profiles in unexplained infertility.

Glycobiology·2026
Same author

<i>Fusobacterium nucleatum</i> Lipopolysaccharides Disrupt the Interaction of Siglec‑7 to Sialoglycans Expressed on Mammalian Cells.

JACS Au·2026
Same author

<i><b>Akkermansia muciniphila</b></i> <b>modulates intestinal mucus composition to counteract high-fat diet-induced obesity in mice</b>.

Gut microbes·2026
Same author

<i>Fusobacterium nucleatum</i> Lipopolysaccharides O‑Antigen Defines a Novel Siglec‑7 Binding Epitope.

JACS Au·2025
Same author

A structural basis for the strain-dependent UDP-sugar specificity of glycosyltransferase C from the Limosilactobacillus reuteri accessory secretion system.

Acta crystallographica. Section D, Structural biology·2025
Same author

Cell surface polysaccharides in the gut microbiota: occurrence, structure and role.

Gut microbes·2025

This study introduces a flow cytometry method to analyze lectin binding to mucin carbohydrates. This technique efficiently determines carbohydrate-binding specificities for studying glycan-protein interactions.

Area of Science:

  • Biochemistry
  • Glycobiology
  • Analytical Chemistry

Background:

  • Mucin O-glycosylated domains are rich in carbohydrates (50-80%) and adopt expanded conformations.
  • Understanding carbohydrate-protein interactions is crucial in various biological processes.

Purpose of the Study:

  • To develop and validate a flow cytometry (FCM) method for assessing lectin binding specificities to mucin carbohydrates.
  • To provide a high-throughput tool for analyzing glycan-lectin interactions.

Main Methods:

  • Immobilization of biotinylated mucin onto streptavidin-coated beads.
  • Monitoring of mucin sugar chain binding using fluorescein-labeled lectins.
  • Determination of lectin specificities toward specific biotinylated glycans as controls.

More Related Videos

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs
04:15

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
08:21

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Published on: September 15, 2021

Related Experiment Videos

Last Updated: Jun 12, 2026

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
09:57

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs
04:15

A Flow Cytometry-Based High-Throughput Technique for Screening Integrin-Inhibitory Drugs

Published on: February 2, 2024

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues
08:21

High-Dimensionality Flow Cytometry for Immune Function Analysis of Dissected Implant Tissues

Published on: September 15, 2021

Main Results:

  • The developed FCM method successfully determined carbohydrate-binding specificities of lectins to mucin.
  • GC-MS and MALDI-ToF were used to characterize the major mucin sugar chains.
  • The method demonstrated flexibility, speed, sensitivity, and high-throughput capability.

Conclusions:

  • Flow cytometry is a valuable tool for studying diverse interactions between glycans and proteins.
  • This FCM method offers an efficient approach to characterize lectin-mucin binding specificities.
  • The technique facilitates high-throughput analysis of glycan-binding proteins.