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Non-invasive Imaging and Analysis of Cerebral Ischemia in Living Rats Using Positron Emission Tomography with 18F-FDG
Published on: December 28, 2014
Intravital oxygen radical imaging in normal and ischemic rat cortex
Yong Wang1, Seiji Yamamoto, Atsuo Miyakawa
1Photon Medical Research Center, Department of Neurosurgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
Neurosurgery
|June 19, 2010
Summary
Reactive oxygen species (ROS) generation increased during early reperfusion, not ischemia, in brain tissue. This study developed an intravital fluorescence imaging method to accurately measure ROS production in situ.
Area of Science:
- Neuroscience
- Biochemistry
- Medical Imaging
Background:
- Cerebral ischemia/reperfusion injury involves complex biochemical processes.
- Reactive oxygen species (ROS) play a critical role in the pathophysiology of ischemia/reperfusion.
- Accurate measurement of ROS generation in vivo is crucial for understanding these processes.
Purpose of the Study:
- To investigate reactive oxygen species (ROS) generation during cerebral ischemia and reperfusion using intravital fluorescence imaging.
- To develop and validate a method for correcting fluorescence intensity changes caused by hemoglobin absorption during ischemia.
Main Methods:
- Intravital fluorescence imaging with MitoSOX and hydroxyphenyl fluorescein in anesthetized rats undergoing forebrain ischemia.
- Correction of fluorescence intensity (FI) for hemoglobin absorption using fluoromicrospheres.
- Confocal microscopy was used to capture images.
Main Results:
- Corrected ROS indicator fluorescence showed no change during ischemia, unlike raw data.
- Significant increases in ROS (superoxide radical and hydroxyl radical) were observed in early reperfusion.
- ROS increases were localized to areas adjacent to arteries and hydroxyl radical was scavenged by edaravone.
Conclusions:
- ROS production significantly increases during the early reperfusion phase after cerebral ischemia, not during ischemia itself.
- The developed intravital fluorescence imaging method is effective for in situ investigation of intracellular ROS production.
- Findings highlight the spatial and temporal dynamics of ROS generation in cerebral ischemia/reperfusion.
