Synthesis of 4',7-diacetoxyapigenin and its apoptotic induction in human Hep G2 cells
Keyong Xu1, Feng Liu, Benguo Liu
1College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China; E-Mail: fezhning@scut.edu.cn (Z.N.).
Abstract:
In this study, 4',7-diacetoxyapigenin [4-(7-acetoxy-5-hydroxy-4-oxo-4H-chromen-2-yl) phenyl acetate] was synthesized for the first time. Its chemical structure was identified by UV, ESI-MS, (1)H and (13)C-NMR. It could inhibit the proliferation of Hep G2 cells in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treatment by 4',7-diacetoxyapigenin, phosphatidylserine of Hep G2 cells could significantly translocate to the surface of the membrane. The increase of an early apoptotic population was observed by both annexin-FITC and PI staining. It was concluded that 4',7-diacetoxyapigenin not only induced cells to enter into apoptosis, but also affected the progress of the cell cycle.
