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Updated: Jun 12, 2026
Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
Published on: September 26, 2025
Lipoglycans of Mycobacterium tuberculosis: isolation, purification, and characterization
Libin Shi1, Jordi B Torrelles, Delphi Chatterjee
1Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.
Abstract:
In this chapter, we describe in detail the steps involved in isolation and characterization of lipoglycans from Mycobacterium tuberculosis and Mycobacterium smegmatis. In addition, procedures involved in structural analysis such as immunoblotting with mAb CS-35 or CS-40, gas chromatography, gas chromatography/mass spectrometry, nuclear magnetic resonance spectroscopy, and endoarabinanase digestion followed by high-pH anion exchange chromatography and two-dimensional gel electrophoresis are presented.
