Related Experiment Video
Updated: Jun 12, 2026

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
Published on: November 8, 2016
[Development and evaluation of whole-genome DNA microarray of Vibrio parahaemolyticus]
Haihong Han1, Xiaotao Zeng, Shengjun Yin
1Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, China. shanlushanlu@163.com
Objective:
We developed and evaluated a whole-genome DNA microarray of Vibrio parahaemolyticus.
Methods:
Based on the genomic sequences of V. parahaemolyticus, we chose a total number of 4770 genes, amplified them by PCR with specific primers, purified the PCR products and printed them onto glass slides. We performed two sets of hybridizations by the method of two-fluorescence comparative hybridization to evaluate the microarray quality, followed by PCR method to validate parts of microarray results.
Results:
Microarray hybridization results were completely consistent with theory expectations and PCR verification results.
Conclusion:
We successfully developed a batch of good quality whole-genome DNA microarrays of V. parahaemolyticus, built up a method of microarray-based comparative genomic hybridization of V. parahaemolyticus and a set of microarray data analysis standard method.
Related Concept Videos
DNA Microarrays
Automated Microbial Diagnostics
Modern Molecular Taxonomy
Genomic DNA in Prokaryotes
Genomic Diversity in Bacteria
Although bacterial genomes are much...

