[On the possibility to distinguish mitotypes in mixed biological specimens based on selective amplification of...

A method is proposed allowing to identify individual mitotypes in mixed preparation of mitochondrial DNA (mtDNA). Analysis of ambiguous localization on a standard electrophoregram during typing of a mixture of individual mtDNA was used to simulate conditions for selective amplification of one of the components, i.e. for its generation as a monoproduct of the polymerase chain reaction, that was followed by its sequenation and identification of individual mitotype. The possibility to use selective amplification of mtDNA sequences for differentiation between mitotypes in mixed samples is discussed with reference to its application in forensic medical examination.