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Related Experiment Video

Updated: Jun 12, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

Robust, high-throughput solution for blood group genotyping.

Gaelle C Le Goff1, Jean-Charles Brès, Dominique Rigal

  • 1Equipe Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, CNRS 5246 ICBMS, Université Lyon 1, Bâtiment CPE, 43 bd du 11 novembre 1918, 69622 Villeurbanne Cedex, France.

Analytical Chemistry
|June 22, 2010
PubMed
Summary
This summary is machine-generated.

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A new automated method, HiFi-Blood 96, enables high-throughput blood group genotyping for improved safety. This assay accurately identifies key blood group antigens, enhancing blood qualification processes.

Area of Science:

  • Transfusion Medicine
  • Genetics
  • Clinical Diagnostics

Background:

  • Increasing blood transfusions necessitate advanced safety measures.
  • Current blood qualification methods require integration of high-throughput, multiparametric assays.

Purpose of the Study:

  • To introduce HiFi-Blood 96, a novel automated method for extended blood group genotyping.
  • To assess the performance of HiFi-Blood 96 in identifying clinically significant blood group antigens.

Main Methods:

  • Development of genotyping assays for six single-nucleotide polymorphisms (SNPs) linked to Kell, Kidd, Duffy, and MNS blood group systems.
  • Validation of the HiFi-Blood 96 method using a panel of 293 blood samples.
  • Comparison of HiFi-Blood 96 genotypes with phenotypes determined by standard serologic techniques.

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Last Updated: Jun 12, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
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Published on: November 19, 2013

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Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

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Main Results:

  • Excellent correlation between genotypic and phenotypic results for five out of six SNPs.
  • High matching percentages for Kell (100%), Kidd (98.9%), Duffy (97.7%), and MNS3/MNS4 (97.4%) systems.
  • A concordance of 83.3% was achieved for the MNS1/MNS2 polymorphism.

Conclusions:

  • The HiFi-Blood 96 method offers a robust and automated solution for extended blood group genotyping.
  • This approach can significantly upgrade blood qualification center facilities.
  • The assay demonstrates high accuracy for most clinically important blood group antigens, supporting enhanced transfusion safety.