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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Cobinamide-based cyanide analysis by multiwavelength spectrometry in a liquid core waveguide.

Jian Ma1, Purnendu K Dasgupta, William Blackledge

  • 1Department of Chemistry and Biochemistry, University of Texas, 700 Planetarium Place, Arlington, Texas 76019-0065, USA.

Analytical Chemistry
|June 22, 2010
PubMed
Summary

A new cyanide analyzer uses cobinamide chemistry for sensitive detection. This method offers a low limit of detection and high throughput for analyzing cyanide in various samples.

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Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Biochemistry

Background:

  • Cyanide is a highly toxic compound requiring accurate and sensitive detection methods.
  • Existing analytical techniques for cyanide may suffer from interferences and limited dynamic range.
  • Cobinamide chemistry offers a novel approach for selective cyanide detection.

Purpose of the Study:

  • To develop and validate a novel cyanide analyzer utilizing cobinamide chemistry.
  • To achieve a low limit of detection (LOD) and a wide linear dynamic range for cyanide quantification.
  • To assess the analyzer's applicability in real-world samples and its resistance to common interferences.

Main Methods:

  • Simultaneous reagent and sample injection into a liquid core waveguide (LCW) flow cell.
  • Detection using a white light emitting diode and a fiber-optic charge coupled device (CCD) spectrometer.
  • Multiwavelength detection for artifact correction and drift compensation.

Main Results:

  • The developed cyanide analyzer achieved an LOD of 30 nM and a linear dynamic range up to 10 microM.
  • High sample throughput of 38 samples/h with a response time of <95 s for 1% carryover.
  • Demonstrated applicability in analyzing cyanide in fruit seed extracts and breath air samples with good spike recoveries (91-108%).

Conclusions:

  • The novel cobinamide-based cyanide analyzer provides sensitive, selective, and rapid detection.
  • Multiwavelength detection effectively corrects for artifacts and drift, enhancing reliability.
  • The analyzer is suitable for diverse applications, including environmental monitoring and biological sample analysis.