[Establishment and evaluation of experimental sepsis mouse model]

Area of Science:

• Hematology
• Immunology
• Infectious Diseases

Context:

• Malignant hematological diseases (leukemia, lymphoma, aplastic anemia) often lead to severe infections like sepsis.
• Sepsis is difficult to diagnose and has a high mortality rate, particularly in immunocompromised patients.
• Current understanding of sepsis pathogenesis in the context of hematological malignancies requires further in vivo investigation.

Purpose:

• To establish a reliable experimental mouse model for sepsis.
• To mimic the clinical presentation and pathological features of sepsis in a controlled setting.
• To provide a foundation for studying sepsis pathogenesis and developing therapeutic interventions in relevant disease contexts.

Summary:

• A caecal ligation and puncture (CLP) method was employed to induce sepsis in mice.
• Mice subjected to CLP exhibited high mortality, significant weight loss, and elevated inflammatory mediators (C5a, IL-6, TNF-alpha, IFN-gamma).
• CLP induced lymphocyte apoptosis in the thymus and mesenteric lymph nodes, alongside pathological damage to the thymus and spleen.

Impact:

• The established mouse model accurately replicates clinical sepsis symptoms and pathological changes.
• This model serves as a valuable tool for in vivo research into the mechanisms and treatment of sepsis.
• Facilitates the exploration of sepsis interventions in conjunction with malignant hematological diseases.