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A Rapid Strategy for the Isolation of New Faustoviruses from Environmental Samples Using Vermamoeba vermiformis
Published on: June 4, 2016
Ruegeria faecimaris sp. nov., isolated from a tidal flat sediment
Ki-Hoon Oh1, Yong-Taek Jung1, Tae-Kwang Oh1
1Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Republic of Korea.
Abstract:
A Gram-negative-staining, non-motile and rod-shaped bacterial strain, HD-28(T), was isolated from a tidal flat of the Yellow Sea, Korea. Strain HD-28(T) grew optimally at pH 7.0-8.0 and 30 °C in the presence of 2-3 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain HD-28(T) was most closely related to species of the genus Ruegeria and exhibited 95.5-96.9 % 16S rRNA gene sequence similarity to the type strains of Ruegeria species. A neighbour-joining phylogenetic tree based on gyrB gene sequences also showed that strain HD-28(T) fell within the cluster comprising recognized species of the genus Ruegeria, showing 77.5-83.9 % sequence similarity. Strain HD-28(T) contained Q-10 as the predominant ubiquinone and C(18 : 1)ω7c as the major fatty acid. The major polar lipids detected in strain HD-28(T) were phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid and two unidentified lipids. The DNA G+C content was 57.9 mol%. Differential phenotypic properties, together with phylogenetic distinctiveness, demonstrated that strain HD-28(T) could be distinguished from recognized species of the genus Ruegeria. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain HD-28(T) is considered to represent a novel species of the genus Ruegeria, for which the name Ruegeria faecimaris sp. nov. is proposed. The type strain is HD-28(T) ( = KCTC 23044(T) = CCUG 58878(T)).
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