Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Step-like concave retroreflector for single pulse Compton backscattering.

Optics express·2025
Same author

Characteristics of Mandibular Canal Branches Related to Nociceptive Marker.

Journal of dental research·2021
Same author

Phase control of a z-current-driven plasma column.

Physical review. E·2020
Same author

Non-uniformity smoothing of direct-driven fuel target implosion by phase control in heavy ion inertial fusion.

Scientific reports·2019
Same author

Peculiar behavior of Si cluster ions in a high-energy-density solid Al plasma.

Physical review. E·2019
Same author

Differential count and time-course analysis of the cellular composition of coelomocyte aggregate of the Japanese sea cucumber Apostichopus japonicus.

Fish & shellfish immunology·2016
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
08:57

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Published on: June 21, 2011

Multispectral computed-tomography microscope for 3-D material analysis.

O Nakamura, S Kawata, S Minami

    Applied Optics
    |June 22, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study reconstructs 3D density distributions of materials using multispectral imaging and optical microscope tomography. This novel approach enables detailed microscopic analysis of complex biological samples.

    More Related Videos

    A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
    08:47

    A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

    Published on: March 15, 2021

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
    08:46

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

    Published on: April 13, 2016

    Related Experiment Videos

    Last Updated: Jun 12, 2026

    High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT
    08:57

    High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

    Published on: June 21, 2011

    A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography
    08:47

    A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

    Published on: March 15, 2021

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages
    08:46

    Using Synchrotron Radiation Microtomography to Investigate Multi-scale Three-dimensional Microelectronic Packages

    Published on: April 13, 2016

    Area of Science:

    • Microscopy
    • Image Processing
    • Materials Science

    Background:

    • Accurate 3D material characterization is crucial for understanding biological structures.
    • Existing methods may lack the resolution or specificity for complex samples.

    Purpose of the Study:

    • To develop and demonstrate a method for reconstructing 3D density distributions of individual materials.
    • To combine multispectral image processing with optical microscope tomography for enhanced analysis.

    Main Methods:

    • Development of microscope optics and numerical methods for 3D reconstruction.
    • Integration of multispectral image processing techniques.
    • Utilization of a previously developed optical microscope tomography system.
    • Application of an experimental apparatus for data acquisition.

    Main Results:

    • Successful reconstruction of three-dimensional density distributions for individual materials.
    • Demonstration of the method's efficacy using biological samples.
    • Validation of the combined approach for detailed microscopic observation.

    Conclusions:

    • The combined approach of multispectral imaging and optical microscope tomography effectively reconstructs 3D material densities.
    • This technique offers a powerful tool for analyzing complex biological samples at a microscopic level.