Related Experiment Video
Updated: Jun 12, 2026
07:57
Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin
Published on: August 25, 2022
Brain alpha- and beta-globin expression after intracerebral hemorrhage
Yangdong He1, Ya Hua, Jin-Yul Lee
1Department of Neurosurgery, University of Michigan, Ann Arbor, Michigan.
Translational Stroke Research
|June 22, 2010
Summary
Intracerebral hemorrhage (ICH) increases hemoglobin (Hb) expression in brain neurons and glia. This suggests a protective role for neuronal Hb in buffering heme during clot resolution after brain injury.
Area of Science:
- Neuroscience
- Biochemistry
- Cell Biology
Background:
- Hemoglobin (Hb) is found in cerebral neurons and can be induced after cerebral ischemia.
- The role of Hb in the brain following intracerebral hemorrhage (ICH) is not fully understood.
Purpose of the Study:
- To investigate the effects of ICH on hemoglobin (Hb), alpha-globin (HbA), and beta-globin (HbB) mRNA and protein levels in the brain.
- To determine the cellular localization of Hb in the brain after ICH.
Main Methods:
- In vivo study using Sprague-Dawley rats subjected to sham or ICH procedures.
- In vitro study using cultured rat brain cells exposed to hemin and deferoxamine.
- Measurement of HbA, HbB, Hb, and hemopexin mRNA and protein levels.
Main Results:
- HbA, HbB, and Hb are primarily expressed in neurons, with lower levels in astrocytes and microglia.
- ICH increased HbA, HbB, and Hb expression in the perihematomal zone, localized in neurons and glia.
- Hemin treatment increased neuronal HbA, HbB, and hemopexin mRNA, while deferoxamine reduced hemin-induced Hb expression.
Conclusions:
- ICH induces HbA and HbB expression in the brain, particularly in neurons and glia.
- Increased Hb expression following ICH may serve a neuroprotective function by buffering free heme.
- Findings highlight a potential endogenous defense mechanism in the brain against heme toxicity.
Related Concept Videos
01:29Hemorrhagic Stroke ll: Pathophysiology
A hemorrhagic stroke develops when a cerebral blood vessel ruptures, allowing blood to escape into the surrounding brain tissue, as in intracerebral hemorrhage (ICH), or into the subarachnoid space, as in subarachnoid hemorrhage (SAH). Because the skull is a rigid compartment, the sudden presence of extravascular blood rapidly increases intracranial pressure and compresses adjacent neural structures, leading to immediate tissue injury and impaired cerebral perfusion.Mass Effect and Primary...
01:17Hemorrhagic Stroke l: Introduction
A hemorrhagic stroke is an acute neurological event that occurs when a weakened cerebral blood vessel ruptures, allowing blood to accumulate within or around the brain. The sudden release of blood forms a focal hematoma that increases intracranial pressure, displaces neural tissue, and can obstruct cerebrospinal fluid pathways. These effects may be compounded by intraventricular extension of the hemorrhage, cerebral edema, or compression of adjacent structures, all of which contribute to...