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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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The outcome of any hypothesis testing leads to rejecting or not rejecting the null hypothesis. This decision is taken based on the analysis of the data, an appropriate test statistic, an appropriate confidence level, the critical values, and P-values. However, when the evidence suggests that the null hypothesis cannot be rejected, is it right to say, 'Accept' the null hypothesis?
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Hückel's Rule Diagram of π MOs: Frost Circle

The Frost circle or the inscribed polygon method is a graphical method for determining the relative energies of π molecular orbitals (MOs) for planar, fully conjugated, and monocyclic compounds. This method was first described by A. A. Frost and Boris Musulin in 1953.
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The F-test is used to compare two sample variances to each other or compare the sample variance to the population variance. It is used to decide whether an indeterminate error can explain the difference in their values. The underlying assumptions that allow the use of the F-test include the data set or sets are normally distributed, and the data sets are independent of each other. The test statistic F is calculated by dividing one variance by another. In other words, the square of one standard...
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Biasing a Junction Field Effect Transistor (JFET) is crucial for setting operational parameters and ensuring efficient functioning in electronic circuits. JFETs are characterized by using a single carrier type in N-channel or P-channel configurations, where the channel is surrounded by PN junctions. These junctions are central to the device's ability to control current flow.
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Health Information Technology and Healthcare Information System

Health Information Technology (HIT)
Health Information Technology, commonly called HIT, integrates advanced information systems and technology in healthcare settings. Its primary functions include:

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Loss of fragile histidine triad gene expression in advanced lung cancer is consequent to allelic loss at 3p14 locus and promoter methylation.

Molecular cancer research : MCR·2006
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Updated: Jun 12, 2026

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity
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Published on: January 15, 2014

FHIT: doubts are clear now.

Anjilna Wali1

  • 1Cell and Molecular Biology Laboratory, Department of Biotechnology, University of Pune, Pune, Maharashtra, India. anjlina_wali@rediffmail.com

Thescientificworldjournal
|June 22, 2010
PubMed
Summary

The fragile histidine triad (FHIT) gene acts as a tumor suppressor. Its loss of function is linked to cancer, and restoring FHIT can inhibit tumor growth.

Area of Science:

  • Molecular biology
  • Cancer genetics

Background:

  • The fragile histidine triad (FHIT) gene, located on chromosome 3p, is recognized as a tumor suppressor.
  • Alterations in FHIT function are implicated in various cancers.

Purpose of the Study:

  • To investigate the role of FHIT gene alterations in cancer development.
  • To explore the potential of FHIT restoration in suppressing tumorigenicity.

Main Methods:

  • Analysis of loss of heterozygosity in FHIT gene sections.
  • Methylation analysis of the FHIT gene promoter region.
  • Evaluation of FHIT restoration effects in cell lines and nude mice models.

Main Results:

  • Loss of heterozygosity and promoter methylation are identified as early genetic alterations in FHIT.

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  • Restoration of FHIT function in cancer cells suppressed tumor formation in vitro and in vivo.
  • Fhit protein interacts with various cellular pathways, influencing apoptosis.

    • Conclusions:

    • FHIT gene alterations are critical early events in tumorigenesis.
    • FHIT gene restoration presents a potential therapeutic strategy for cancer treatment.
    • FHIT's role in apoptosis highlights its significance in cell fate determination.