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Published on: October 21, 2015
Function analysis of mesenchymal Bcor in tooth development by using RNA interference.
Jinglei Cai1, Sungwook Kwak, Jong-Min Lee
1Division in Anatomy and Developmental Biology, Department of Oral Biology, Research Center for Orofacial Hard Tissue Regeneration, Brain Korea 21 Project, Oral Science Research Center, College of Dentistry, Yonsei Center of Biotechnology, Yonsei University, Seoul, South Korea.
Bcor (BCL-6 interacting corepressor) is crucial for tooth development. Its absence in dental mesenchymal cells causes dentinogenesis defects and root development issues.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- Tooth development involves complex epithelial-mesenchymal interactions.
- BCL-6 interacting corepressor (Bcor) function in tooth development is largely unknown.
- BCOR mutations cause oculofaciocardiodental syndrome in humans.
Purpose of the Study:
- Investigate Bcor expression patterns during mouse molar development.
- Determine the role of Bcor in tooth organogenesis.
Main Methods:
- Examined Bcor expression during embryonic mouse molar development (E11.5).
- Utilized lentivirus-mediated RNA interference for Bcor knockdown in dental mesenchymal cells (E14.5).
Main Results:
- Bcor is expressed in both dental epithelium and mesenchyme at E11.5.
- Bcor knockdown in mesenchymal cells led to dentinogenesis defects.
- Silencing Bcor resulted in retarded tooth root development.
Conclusions:
- Bcor plays critical roles in early tooth development, particularly within the dental mesenchyme.
- Further research is needed to elucidate Bcor's function in the dental epithelium.
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