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Updated: Jun 12, 2026

In Vitro Phagocytosis of Myelin Debris by Bone Marrow-Derived Macrophages

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Published on: December 30, 2017

[The macrophage electrophoretic mobility LAD test - a diagnostic method for multiple sclerosis.].

H Meyer-Rienecker¹, H L Jenssen, H Köhler

¹Neurologische Abteilung der Nervenklinik und Forschungsabteilung Immunologie des Bereiches Medizin, Universität Rostock, Germany.

Journal of Neurology

|June 22, 2010

Summary

The macrophage migration inhibition test (MEM-LAD) shows significantly higher inhibition in multiple sclerosis (MS) patients, suggesting a cellular immune response. This test may help identify familial predispositions to MS.

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Area of Science:

Immunology
Neurology
Cellular Biology

Context:

Cellular immune reactions involve sensitized lymphocytes releasing macrophage slowing factor (MSF) upon antigen interaction.
Macrophage migration inhibition is observed in neurological diseases with parenchymal destruction.
The macrophage migration inhibition test (MEM-LAD) is a modified assay for enhanced differentiation.

Purpose:

To differentiate neurological diseases using the modified MEM-LAD test.
To investigate cellular immune responses in multiple sclerosis (MS) and other neurological conditions.
To explore potential familial (genetic) predispositions in MS.

Summary:

The MEM-LAD test revealed significantly higher macrophage mobility inhibition in MS patients (94.7%) compared to normals (55.1%) and other neurological diseases (47.8%).

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No significant differences were noted based on disease course, duration, or immunosuppressive therapy.

Elevated inhibition in MS relatives (78.5%) and a monozygotic twin suggests a familial/genetic disposition.

Impact:

The MEM-LAD test demonstrates potential for diagnosing MS and identifying individuals at risk.
Findings suggest both endogenic metabolic and early-life exogenic factors contribute to MS etiology.
The suppressive action of linoleic acid identified via the MEM-LAD test may inform new therapeutic strategies for MS.