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Updated: Jun 12, 2026
High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model
Published on: August 16, 2019
Posttranslationally modified peptides efficiently mimicking neoantigens: a challenge for theragnostics of autoimmune
Francesca Nuti1, Elisa Peroni, Feliciana Real-Fernández
1Laboratory of Peptide and Protein Chemistry and Biology, Polo Scientifico e Tecnologico, University of Florence, Via della Lastruccia 3/13, I-50019 Sesto Fiorentino (FI), Italy.
Abstract:
We report the design, synthesis, and immunological evaluation of a series of glycopeptide analogues of the previously described antigenic probe CSF114(Glc), with the aim of understanding the importance of N-glycosylation on Asn residue in multiple sclerosis antibody recognition. The glucopeptide, characterized by a β-turn conformation which is fundamental for a correct presentation of the epitope, has been modified by introducing various natural glycoamino acids in position 7. The new glycopeptides were evaluated by measuring the IgG and IgM antibody titer in multiple sclerosis patients' and normal blood donors' sera. Moreover, we achieved the efficient synthetic strategy of new Asn derivative bearing N-acetylneuraminic acid (Neu5Ac), linked by an N-glycosidic bond, on the side chain of the Asn residue orthogonally protected for Fmoc/tBu SPPS.
