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Resveratrol induces cell-cycle disruption and apoptosis in chemoresistant B16 melanoma

Grégory Gatouillat1, Emilie Balasse, Débora Joseph-Pietras

  • 1Faculty of Pharmacy, Department of Biochemistry and Molecular Biology, URCA, Reims, France.

Journal of Cellular Biochemistry
|June 22, 2010
PubMed

Insights

Resveratrol inhibits the growth of chemoresistant melanoma by halting cell cycle progression and inducing apoptosis. This natural compound also enhances doxorubicin

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Resveratrol, a polyphenol, exhibits chemopreventive properties.
  • Chemotherapeutic resistance, particularly to doxorubicin, is a significant clinical challenge in melanoma treatment.

Purpose of the Study:

  • To investigate the efficacy of resveratrol against doxorubicin-resistant B16 melanoma.
  • To elucidate the mechanisms underlying resveratrol's anti-cancer effects.
  • To evaluate resveratrol's therapeutic potential in vivo.

Main Methods:

  • Cell culture of doxorubicin-resistant B16 melanoma (B16/DOX) cells.
  • Resveratrol treatment and assessment of cell viability (IC50 determination).
  • Cell cycle analysis, Western blotting for cyclin D1/cdk4 and p53 expression.
  • Apoptosis assays (nuclear morphology, DNA fragmentation).
  • In vivo studies using a mouse melanoma model.

Main Results:

  • Resveratrol inhibited B16/DOX cell growth with an IC50 of 25 microM.
  • Resveratrol induced G1-S phase arrest by down-regulating cyclin D1/cdk4 and up-regulating p53.
  • Resveratrol triggered apoptosis in B16/DOX cells.
  • Resveratrol potentiated doxorubicin cytotoxicity in chemoresistant melanoma.
  • In vivo, resveratrol reduced tumor growth and prolonged survival in mice.

Conclusions:

  • Resveratrol demonstrates significant anti-cancer activity against chemoresistant melanoma.
  • Resveratrol's mechanism involves cell cycle arrest and apoptosis induction.
  • Resveratrol holds potential as a therapeutic agent for chemoresistant melanoma, alone or in combination therapy.

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