Related Experiment Video
Updated: Jun 12, 2026

The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
Chromosomal integration of a synthetic expression control sequence achieves poly-gamma-glutamate production in a
Chuan-Mei Yeh1, Jyh-Perng Wang, Shih-Ching Lo
1Dept. of Food Science and Biotechnology, National Chung-Hsing University, Taichung, Taiwan, R.O. China. cmyeh@nchu.edu.tw
Abstract:
Poly-gamma-glutamate (gamma-PGA) has applications in food, medical, cosmetic, animal feed, and wastewater industries. Bacillus subtilis DB430, which possesses the gamma-PGA synthesis ywsC-ywtAB genes in its chromosome, cannot produce gamma-PGA. An efficient synthetic expression control sequence (SECS) was introduced into the upstream region of the ywtABC genes, and this resulted in gamma-PGA-producing B. subtilis mutant strains. Mutant B. subtilis PGA6-2 stably produces high levels of gamma-PGA in medium A without supplementation of extra glutamic acid or ammonium chloride. The mutant B. subtilis PGA 6-2 is not only a gamma-PGA producer, but it is also a candidate for the genetic and metabolic engineering of gamma-PGA production.
Related Concept Videos
Coordination of Gene Expression Processes in Bacteria
Reporter Genes
Commonly used reporter...

