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Updated: Jun 12, 2026

A High-throughput Calcium-flux Assay to Study NMDA-receptors with Sensitivity to Glycine/D-serine and Glutamate
Published on: July 10, 2018
[NMDA-dependent increase of exracellular arginine level in the nucleus accumbens is mediated by NO-synthase
Abstract:
In Sprague-Dawley rats, by means of in vivo microdialysis combined with HPLC analysis it was shown that intra-accumbal infusion of NMDA (10 microM, 100 microM, 1000 microM) dose-dependently increased the local dialysate level of arginine (an NO-synthase substrate). This increase was prevented by intraaccumbal infusion of 50 microM MK-801, an NMDA receptor antagonist, and it was significantly reduced by intra-accumbal infusion of 500 microM N-nitroarginine, an NO-synthase inhibitor. The data obtained indicate that extracellular level of arginine in the n. accumbens is regulated by NMDA receptors which act partially via NO-synthase activation.
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