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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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Related Experiment Video

Updated: Jun 12, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

MicroRNAs: more than a role in plant development?

¹Dpto. Biología de Plantas, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040-Madrid, Spain.

Molecular Plant Pathology

|June 23, 2010

Summary

Plant microRNAs (miRNAs) regulate growth, development, and stress responses. Potyviruses can disrupt these plant miRNAs, altering host gene expression.

Area of Science:

Molecular Biology
Plant Science
Genetics

Background:

Eukaryotic small RNAs, including microRNAs (miRNAs), are crucial non-coding RNAs involved in gene regulation.
miRNAs are 21-25 nucleotide RNAs processed by Dicer and guide target mRNA cleavage or translational repression.
Natural miRNA targets often encode transcription factors essential for plant development and stress responses.

Purpose of the Study:

To explore the role of plant microRNAs in development and stress responses.
To investigate the interaction between plant viruses and the miRNA pathway.

Main Methods:

Analysis of small RNA populations in plants.
Identification and characterization of miRNA targets.
Investigating viral interference with miRNA-mediated gene silencing.

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A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

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Last Updated: Jun 12, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

A Bioinformatics Pipeline to Accurately and Efficiently Analyze the MicroRNA Transcriptomes in Plants

Published on: January 21, 2020

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

Main Results:

Plant miRNAs regulate key developmental processes and physiological responses to stress.
Potyviruses, a major group of plant RNA viruses, interfere with miRNA-guided cleavage.
Viral interference modulates host gene expression, impacting cellular processes.

Conclusions:

MicroRNAs are vital regulators of plant biology, influencing growth, development, and stress adaptation.
Viral manipulation of the miRNA pathway represents a significant strategy for host gene expression modulation by plant viruses.