Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Response to correspondence by Johan Øvrevik.

Indoor air·2014
Same author

Effect of relative humidity on the aerosolization and total inflammatory potential of fungal particles from dust-inoculated gypsum boards.

Indoor air·2013
Same author

Optical tomography of the in vivo human lens: three-dimensional visualization of cataracts.

Journal of biomedical optics·2012
Same author

Rapid observation of unfixed, unstained human skin biopsy specimens with confocal microscopy and visualization.

Journal of biomedical optics·2012
Same author

Precision and Reproducibility of Measurements of Human Corneal Thickness with Rapid Optical Low-Coherence Reflectometry (OLCR).

Journal of biomedical optics·2012
Same author

Early development of optical low-coherence reflectometry and some recent biomedical applications.

Journal of biomedical optics·2012
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
12:59

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

Three-dimensional microscopy: introduction by the feature editors.

T C Strand, E W Hansen, B R Masters

    Applied Optics
    |June 23, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This issue explores advances in 3-D microscopy, highlighting its applications and the motivations for this specialized collection. Discover the latest in three-dimensional imaging techniques.

    More Related Videos

    Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
    10:51

    Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

    Published on: April 16, 2014

    A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
    08:46

    A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

    Published on: June 23, 2023

    Related Experiment Videos

    Last Updated: Jun 12, 2026

    Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
    12:59

    Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

    Published on: February 28, 2021

    Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
    10:51

    Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

    Published on: April 16, 2014

    A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
    08:46

    A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells

    Published on: June 23, 2023

    Area of Science:

    • Microscopy
    • Biotechnology
    • Imaging Science

    Background:

    • The development of three-dimensional (3-D) microscopy techniques has revolutionized biological and materials science research.
    • Traditional imaging methods often lack the depth and spatial resolution necessary for comprehensive analysis.
    • A growing need exists for advanced imaging solutions that provide a more complete understanding of complex structures.

    Purpose of the Study:

    • To introduce and provide an overview of a special issue dedicated to 3-D microscopy.
    • To highlight the motivations and significance driving the advancements in this field.
    • To showcase recent developments and applications of 3-D microscopy.

    Main Methods:

    • This issue focuses on various 3-D microscopy techniques, including confocal, multiphoton, and light-sheet microscopy.
    • Discussions cover sample preparation, image acquisition, and data processing specific to 3-D imaging.
    • The content emphasizes the practical application and implementation of these advanced microscopy methods.

    Main Results:

    • The featured articles demonstrate the utility of 3-D microscopy in visualizing intricate cellular structures, tissue architectures, and material properties.
    • Key findings showcase enhanced resolution, improved signal-to-noise ratios, and novel analytical capabilities.
    • The presented research underscores the transformative impact of 3-D imaging across diverse scientific disciplines.

    Conclusions:

    • 3-D microscopy offers unparalleled insights into complex biological and material systems.
    • Continued innovation in 3-D imaging technologies promises to accelerate scientific discovery.
    • This special issue serves as a valuable resource for researchers utilizing or interested in advanced three-dimensional microscopy.