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Updated: Jun 12, 2026

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
Published on: April 26, 2024
Continuous affinity chromatography using a magnetically stabilized fluidized bed
1Department of Chemical Engineering University of Pennsylvania Philadelphia, Pennsylvania.
Abstract:
A new processing tool, the magnetically stabilized fluidized bed, offers opportunities for efficient fluid-solids contact.The bubbling and mixing normally seen in a fluidized bed are eliminated, and continuous countercurrent contact is possible. This technique is especially suitable for biochemical separations, where cellular debris would clog an ordinary fixed bed. Preliminary modeling and experimental work on such a system is discussed, along with details of a relatively simple experimental system.
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