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Continuous Liquid-Liquid Extraction of Medium-Chain Fatty Acids from Fermentation Broth Using Hollow-Fiber Membranes

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Published on: August 9, 2024

Multimembrane bioreactor for extractive fermentation.

T Cho¹, M L Shuler

¹School of Chemical Engineering Cornell University Ithaca, NY 14853.

Biotechnology Progress

|June 23, 2010

Summary

This summary is machine-generated.

This study introduces a novel multimembrane reactor for extractive fermentation, successfully producing ethyl alcohol from glucose. The reactor design prevents solvent emulsification, enhancing yeast metabolism and enabling practical solvent use.

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Area of Science:

Biochemical Engineering
Fermentation Technology
Chemical Process Development

Background:

Extractive fermentation aims to overcome product inhibition by removing fermentation products in situ.
Solvent emulsification in fermentation broths is a common challenge, hindering process efficiency and solvent recovery.
Tributyl phosphate (TBP) is a potential extractant but exhibits toxicity to microorganisms.

Purpose of the Study:

To describe and validate a novel multimembrane reactor for extractive fermentation.
To demonstrate the prevention of solvent emulsification in a fermentation system.
To assess the feasibility of using Tributyl phosphate (TBP) as a practical extractant in yeast fermentation.

Main Methods:

A four-layer multimembrane reactor was designed, separating gas, cells, nutrient, and solvent phases.
Ethyl alcohol production from glucose using yeast was conducted in the developed reactor.
Tributyl phosphate (TBP) was employed as the organic extractant.

Main Results:

The multimembrane reactor effectively prevented solvent emulsification in the fermentation broth.
Successful extractive fermentation for ethyl alcohol production was achieved using yeast and TBP.
The absence of emulsification mitigated the toxic effects of TBP on yeast metabolism.

Conclusions:

The developed multimembrane reactor offers a viable solution for preventing solvent emulsification in extractive fermentation.
This technology enables the use of practical solvents like TBP, overcoming previous limitations.
The system shows promise for efficient and scalable bio-production processes.