Related Experiment Video
Updated: Jun 12, 2026
Purification of Platelets from Mouse Blood
Published on: May 7, 2019
[The estimation of the number of platelets by different methods]
Karol Ratomski1, Janusz Zak, Edwina Kasprzycka
1Uniwersytet Medyczny w Bialymstoku, Zakład Laboratoryjnej Diagnostyki Pediatrycznej. karol.cam@interia.pl
Unlabelled:
The commonly available and accurate platelet counts play an important role in the evaluation of haemorrhagic status and in assessing the need for platelet transfusions. We evaluated platelet counting performance by the Sysmex XT-2000i haematology analyser and by the Coulter EPICS XL cytometry using optical, impedance and immunological methods in trombocytopenic patients. Hematology analysers display the high precision and the accuracy of the measurement of the number of platelets in whole blood. However, the measurement of number of platelets can be disturbed by the presence of interference factors such as: microcytic erytrocytes, schizocytes, cryoglobulins, microplatelets and immunological clumps.
The Aim Of The Study:
The comparison of impedance, optical and immunological enumeration methods of platelets by the Sysmex XT-2000i haematology analyser and by the Coulter EPICS XL cytometer.
Results:
The platelet count of all considered methods showed good correlation with the cytometric method.
Conclusions:
Only cytometric method with using anti-CD41 monoclonal antibody allowed to verify the results of enumeration methods of platelets.
