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[Using HPLC-MS/MS method to analyze monohydroxy metabolites of PAHs in urine]
Chuanfeng Huang1, Huifang Yan, Zufei Pan
1National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China. chuanfengh@yahoo.com.cn
Objective:
To establish a HPLC-MS/MS method for the simultaneous determination of 4 monohydroxy metabolites of polycyclic aromatic hydrocarbons (PAHs) in urine, including 1-naphthol, 2-naphthol, 3-phenanthrol and 1-pyrenol).
Methods:
After being treated by enzymatic hydrolysis, urine samples were purified by liquid-liquid extraction, evaporated under nitrogen to constant volume and examined by HPLC-MS/MS. Target compounds were quantified by internal standard calibration curve.
Results:
The reproducibility and precision were improved by internal standard calibration. The recovery of four target compounds were in the range of 89.7%-103.0%. The precision of intra- and inter-batch was 1.0%-4.2% and 1.8%-5.8% respectively. The limits of quantification was 0.2 microg/L for 1-naphthol, 2-naphthol and 1-pyrenol and the limits of quantification was 0.1 microg/L for 3-phenanthrol.
Conclusion:
The sensitivity, reproducibility and recovery of HPLC-MS/MS method were good for the analysis of 1-naphthol, 2-naphthol, 3-phenanthrol and 1-pyrenol in the urine of people on occupational and nonoccupational exposure to PAHs.
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