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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Single molecule biosensing using color coded plasmon resonant metal nanoparticles.

Lehui Xiao1, Lin Wei, Yan He

  • 1Biomedical Engineering Center, College of Chemistry and Chemical Engineering, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082, People's Republic of China.

Analytical Chemistry
|June 24, 2010
PubMed
Summary
This summary is machine-generated.

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This study introduces a novel single-molecule biosensing method using plasmonic nanoparticles (NPs) to detect target molecules by differentiating scattered light colors. The technique achieves high sensitivity and multiplexing capabilities for advanced biological detection.

Area of Science:

  • Nanotechnology
  • Biotechnology
  • Optical Physics

Background:

  • Plasmonic metal nanoparticles (NPs) offer tunable optical properties for biosensing.
  • Current biosensing methods lack sensitivity, quantitation, and cost-effectiveness.
  • Single-molecule detection is crucial for understanding biological processes.

Purpose of the Study:

  • To develop a sensitive, quantitative, and cost-effective single-molecule biosensing method.
  • To utilize color differentiation of scattered light from single NPs and NP aggregates.
  • To enable multiplexed sensing using different types of plasmonic NPs.

Main Methods:

  • Seed-mediated NP growth for monodisperse gold (Au) and gold/silver/gold (Au/Ag/Au) composite NPs.
  • Fast DNA modification for NP probe preparation.

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Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Last Updated: Jun 12, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Published on: November 23, 2015

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

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07:30

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

Published on: March 7, 2018

  • Darkfield microscopy with a color CCD camera for real-time single-NP tracking and analysis.

    • Main Results:

    • Successfully prepared stable, spectrally uniform, and monodisperse Au and Au/Ag/Au NPs.
    • Demonstrated recognition of single target molecule binding via color differentiation of scattered light.
    • Achieved a detection limit of 0.02 pM in a homogeneous assay.
    • Showcased multiplexed sensing using Au NPs and Au/Ag/Au composite NPs as distinct color probes.

    Conclusions:

    • The developed method enables label-free, single-molecule detection without NP separation.
    • The technique offers high sensitivity and potential for multiplexed biosensing.
    • This approach can be applied to immunoassays, single-cell analysis, and biomolecule interaction studies.