Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Equal in death: Ancient genomic analysis of children's early Christian burials.

Science advances·2026
Same author

Genetic relatedness mattered in the co-burial ritual of Neolithic hunter-gatherers.

Proceedings. Biological sciences·2026
Same author

Homo sapiens-specific evolution unveiled by ancient southern African genomes.

Nature·2025
Same author

HLA diversity and signatures of selection in the Maniq, a nomadic hunter-gatherer population in Southern Thailand.

Immunogenetics·2025
Same author

Ancient DNA HLA typing reveals significant shifts in frequency in Europe since the Neolithic.

Scientific reports·2025
Same author

HOMAc: A Parameterization of the Harmonic Oscillator Model of Aromaticity (HOMA) That Includes Antiaromaticity.

The Journal of organic chemistry·2025

Related Experiment Video

Updated: Jun 12, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

Hole mobility and transport mechanisms in lambda-DNA.

Mattias Jakobsson1, Sven Stafström

  • 1Department of Physics, Chemistry and Biology (IFM), Linköping University, SE-581 83 Linköping, Sweden. matja@ifm.liu.se

The Journal of Chemical Physics
|June 24, 2010
PubMed
Summary
This summary is machine-generated.

Charge delocalization in DNA hinders hole mobility, acting as a transport trap. This study reveals how electric fields and temperature affect charge transport in lambda-DNA.

More Related Videos

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

Related Experiment Videos

Last Updated: Jun 12, 2026

Studying DNA Looping by Single-Molecule FRET
11:27

Studying DNA Looping by Single-Molecule FRET

Published on: June 28, 2014

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA
12:05

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Published on: October 1, 2017

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

Area of Science:

  • Molecular Biophysics
  • Computational Biology
  • Charge Transport Phenomena

Background:

  • Understanding charge transport in DNA is crucial for applications in molecular electronics and biosensing.
  • Lambda-DNA serves as a model system for studying charge carrier dynamics within biological macromolecules.

Purpose of the Study:

  • To investigate the impact of charge delocalization on hole mobility in lambda-DNA.
  • To analyze the influence of electric fields and temperature on charge transport dynamics.
  • To identify key bottlenecks limiting charge transport efficiency in DNA.

Main Methods:

  • Utilized a novel model combining Marcus theory and dynamic Monte Carlo simulations.
  • Incorporated charge delocalization over adjacent identical nucleobases into the simulation model.
  • Simulated hole propagation through the DNA sequence to analyze transport pathways.

Main Results:

  • Charge delocalization over multiple nucleobases acts as a significant trap for charge carriers (holes).
  • Hole mobility in lambda-DNA is negatively impacted by these delocalized states.
  • Reported the electric field and temperature dependence of hole mobility.
  • Identified specific sequence segments that impede charge transport.

Conclusions:

  • Delocalized electronic states within DNA sequences can severely limit charge transport efficiency.
  • The developed model provides detailed insights into charge carrier dynamics and bottlenecks in DNA.
  • Findings are relevant for designing DNA-based electronic devices and understanding biological charge transfer processes.