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Related Experiment Video

Updated: Jun 12, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Rat monoclonal antibody specific for MyoD.

Akihito Harada¹, Yasuyuki Ohkawa, Shinpei Ao

¹Department of Epigenetics, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.

Hybridoma (2005)

|June 24, 2010

Summary

Researchers developed a specific monoclonal antibody for MyoD, a key protein in skeletal muscle differentiation. This new tool helps track MyoD expression during muscle development, aiding further research into differentiation mechanisms.

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Method for Whole Mount Antibody Staining in Chick

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Last Updated: Jun 12, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

Method for Whole Mount Antibody Staining in Chick

Method for Whole Mount Antibody Staining in Chick

Published on: February 2, 2009

Area of Science:

Molecular Biology
Developmental Biology
Cell Biology

Background:

Myogenic regulatory factors (MRFs) are crucial for skeletal muscle development.
Myogenic determination 1 (MyoD) acts as a master gene regulating myoblast fate and differentiation.
Understanding MyoD's role requires specific tools to monitor its expression.

Purpose of the Study:

To establish a highly specific monoclonal antibody for MyoD.
To validate the antibody's ability to detect full-length MyoD.
To investigate changes in MyoD expression during skeletal muscle differentiation.

Main Methods:

Development of a monoclonal antibody using the rat medial iliac lymph node method.
Immunoblot analysis to confirm antibody specificity for full-length MyoD.
Immunocytochemical staining to observe MyoD expression during differentiation.

Main Results:

A novel monoclonal antibody specific to MyoD was successfully generated.
The antibody effectively identified full-length MyoD via immunoblotting.
Immunocytochemistry demonstrated dynamic changes in MyoD expression during skeletal muscle differentiation.

Conclusions:

The developed monoclonal antibody is a valuable tool for MyoD research.
This antibody facilitates detailed studies on MyoD's mechanism in skeletal muscle differentiation.
Further investigation into MyoD's role in muscle development is now enabled.