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Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
Published on: November 18, 2017
Effect of liquid storage on sorted boar spermatozoa
M Spinaci1, C Vallorani, D Bucci
1DIMORFIPA, University of Bologna, Bologna, Italy. marcella.spinaci@unibo.it
Theriogenology
|June 24, 2010
Summary
Sexed boar semen sorting damages sperm viability and membrane integrity. Storage further reduces fertility, necessitating further research into mitigating these negative effects.
Area of Science:
- Animal Science
- Reproductive Biology
- Sperm Technology
Background:
- Routine use of sexed boar semen is limited by long sorting times and cryopreservation-induced damage.
- Ensuring the viability and fertility of sorted spermatozoa is crucial for artificial insemination.
Purpose of the Study:
- To evaluate the impact of 24-26 hour storage on sorted boar spermatozoa.
- To assess changes in viability, acrosome status, Hsp70 presence, and in vitro fertilizing ability.
Main Methods:
- Sperm viability assessed using SYBR green/PI staining.
- Acrosome status evaluated with FITC-PNA/PI staining.
- Hsp70 presence analyzed via immunofluorescence and western blot.
Main Results:
- Spermatozoa viability and membrane integrity were negatively affected by sorting and subsequent storage.
- Neither sorting nor storage altered the acrosome reaction rate.
- Storage led to increased Hsp70-negative cells and decreased Hsp70 content.
- Fertilization parameters were negatively impacted by storage post-sorting.
Conclusions:
- Sorting and liquid storage significantly compromise the quality and fertility of boar spermatozoa.
- Further investigation is required to understand and minimize the damaging effects of these procedures.
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