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Updated: Jun 12, 2026

Production of Recombinant PRMT Proteins using the Baculovirus Expression Vector System
Published on: July 17, 2021
Distance between RBS and AUG plays an important role in overexpression of recombinant proteins
Sunil K Berwal1, R K Sreejith, Jayanta K Pal
1Cell and Molecular Biology Laboratory, Department of Biotechnology, University of Pune, Pune 411007, India.
Abstract:
The spacing between ribosome binding site (RBS) and AUG is crucial for efficient overexpression of genes when cloned in prokaryotic expression vectors. We undertook a brief study on the overexpression of genes cloned in Escherichia coli expression vectors, wherein the spacing between the RBS and the start codon was varied. SDS-PAGE and Western blot analysis indicated a high level of protein expression only in constructs where the spacing between RBS and AUG was approximately 40 nucleotides or more, despite the synthesis of the transcripts in the representative cases investigated.
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