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Updated: Jun 12, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

An ELISA-based method to quantify osteocalcin carboxylation in mice.

Mathieu Ferron¹, Jianwen Wei, Tatsuya Yoshizawa

¹Department of Genetics & Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.

Biochemical and Biophysical Research Communications

|June 24, 2010

Summary

Researchers developed a new assay to measure undercarboxylated osteocalcin, a hormone regulating insulin. This method accurately quantifies osteocalcin forms in mouse serum and cell cultures.

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Area of Science:

Endocrinology
Biochemistry
Assay Development

Background:

Osteocalcin is an osteoblast-secreted hormone influencing insulin secretion and sensitivity.
The undercarboxylated form of osteocalcin is hormonally active.
Quantifying osteocalcin forms in mouse serum has been challenging due to a lack of suitable methods.

Purpose of the Study:

To develop and validate a quantitative methodology for measuring total, carboxylated, and uncarboxylated osteocalcin in mouse samples.
To enable reliable assessment of osteocalcin carboxylation status in various experimental settings.

Main Methods:

Development of a triple enzyme-linked immunosorbent assay (ELISA) system.
Validation using warfarin, a known carboxylation inhibitor, in osteoblast cultures.

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Last Updated: Jun 12, 2026

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

Application of the ELISA to mouse serum samples from warfarin-treated and Esp-/- mice.

Main Results:

The developed ELISA system reliably quantifies total, carboxylated, and uncarboxylated osteocalcin.
Warfarin treatment decreased osteocalcin carboxylation in vitro, validating the assay.
Elevated levels of undercarboxylated osteocalcin were detected in serum from warfarin-treated and Esp-/- mice.

Conclusions:

The novel triple ELISA is a reliable tool for assessing osteocalcin carboxylation status in cell cultures and mouse serum.
This method facilitates research into osteocalcin's hormonal activity in different mouse models and experimental systems.