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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Related Experiment Video

Updated: Jun 12, 2026

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy
09:36

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

Published on: October 19, 2018

RNase-assisted RNA chromatography.

Gracjan Michlewski1, Javier F Cáceres

  • 1Medical Research Council Human Genetics Unit, Institute of Genetics and Molecular Medicine, Western General Hospital, Edinburgh EH4 2XU, United Kingdom. Gracjan.Michewski@hgu.mrc.ac.uk

RNA (New York, N.Y.)
|June 24, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces an improved RNA chromatography method using RNases during elution. This technique enhances the detection of RNA-binding proteins by significantly reducing background noise and improving sensitivity.

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Last Updated: Jun 12, 2026

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • RNA chromatography-mass spectrometry is key for identifying RNA-binding proteins.
  • High background from nonspecific protein binding to beads hinders detection sensitivity.
  • Current methods to reduce background compromise physiological conditions and protein complex integrity.

Purpose of the Study:

  • To develop an improved RNA chromatography method for sensitive RNA-binding protein identification.
  • To overcome the limitations of high background noise in existing protocols.
  • To enable more thorough detection of RNA-binding proteins without losing low-affinity binders or complexes.

Main Methods:

  • Utilized RNA chromatography coupled with mass spectrometry.
  • Introduced a cocktail of RNases in the elution step.
  • Optimized washing steps to minimize nonspecific protein binding.

Main Results:

  • Achieved almost complete elimination of background noise.
  • Successfully released proteins specifically associated with the RNA ligand.
  • Enabled more sensitive and thorough detection of RNA-binding proteins.

Conclusions:

  • The improved RNA chromatography method offers enhanced sensitivity for RNA-binding protein detection.
  • RNase-assisted elution effectively removes background, preserving biological relevance.
  • This method facilitates a deeper understanding of RNA-protein interactions.