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Updated: Jun 12, 2026
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
No apparent correlation between Behçet's disease and oxidative stress disturbance
Hayrettin Saglam1, Eser Kaya, Mustafa Cemek
1Department of Cardiology, Acibadem Hospital, Kayseri, Turkey. drsaglam69@yahoo.com
Insights
Behçet's disease patients show increased oxidative stress and myocardial perfusion defects. However, no direct correlation was found between these factors in the inactive disease period.
Area of Science:
- Cardiology
- Rheumatology
- Biochemistry
Background:
- Behçet's disease (BD) is a chronic inflammatory condition affecting multiple systems, notably the cardiovascular system.
- Oxidative stress (OS), an imbalance favoring oxidants, contributes to endothelial dysfunction and cardiovascular complications in BD.
- Understanding the link between OS and myocardial perfusion is crucial for managing cardiovascular risks in BD patients.
Purpose of the Study:
- To investigate the relationship between oxidative stress markers and myocardial perfusion abnormalities in patients with Behçet's disease.
- To assess myocardial perfusion using Tc-99m MIBI SPECT and evaluate OS parameters in BD patients compared to healthy controls.
Main Methods:
- A case-control study involving 27 BD patients and 22 healthy volunteers.
- Myocardial perfusion assessed via Tc-99m MIBI SPECT, analyzing perfusion scores and defect prevalence.
- Blood samples analyzed for oxidative stress markers including malondialdehyde, glutathione, and vitamins.
Main Results:
- Behçet's disease patients exhibited significantly higher levels of oxidative stress markers and a higher prevalence of myocardial perfusion defects compared to controls.
- 51.8% of BD patients showed perfusion defects on scintigraphy; coronary angiography results were normal in those who underwent the procedure.
- No significant correlation was found between myocardial perfusion scores, defect prevalence, and oxidative stress parameters in BD patients.
Conclusions:
- Increased oxidative stress and myocardial perfusion defects are present in Behçet's disease patients during the inactive period.
- Despite the presence of both factors, a direct correlation between oxidative stress levels and the extent of myocardial perfusion defects was not established.
- Myocardial perfusion abnormalities in BD may fluctuate independently of oxidative stress levels in the absence of active inflammation.
Unlabelled:
Behçet's disease (BD) is a chronic, progressive and inflammatory multisystemic disease, that significantly affects the cardiovascular system. Oxidative stress (OS) is a disturbance in oxidant/antioxidant balance in favor of oxidants. The OS that increases acutely and chronically due to the inflammatory process plays an important role in the pathogenesis of the cardiovascular system effects of the disease by causing endothelial dysfunction in vascular structures. The aim of our study was to investigate the relationship between OS and myocardial perfusion, which is based on microvascular dysfunction, in BD.
Material And Method:
Twenty-seven patients with BD (16 M, 11 F, mean age: 38.7 +/- 9.4 years) and 22 healthy volunteers (12 M, 10 F, mean age: 35.8 +/- 6.5 years) participated in our study. Technetium-99m methoxyisobutylisonitrile single photon emission computed tomography (Tc-99m MIBI SPECT) stress-rest test was performed with two-day protocol. Myocardial perfusion scores (summed stress score, summed rest score, summed difference score, fix defect score) and perfusion defect prevalence (stress, rest, ischemic and fixed) were determined as the percentage of left ventricle. Coronary angiography was performed in patients with abnormal myocardial perfusion scintigraphy. For OS analysis, the blood samples were taken immediately before the first imaging procedure and were studied for malondialdehyde, glutathione, nitrite, nitrate, vitamin C, retinol, and carotene.
Results:
In the BD group, a total of 9 patients had abnormal findings in their stress and rest electrocardiography. Perfusion defect in myocardial perfusion scintigraphy was observed in 14 patients (51.8%). Twelve patients accepted coronary angiography, and their results were normal. In the comparison of myocardial perfusion scores, perfusion defect prevalence and OS parameters, there was a significant difference between the BD and control groups. In the BD group, no correlation was observed between myocardial perfusion scores, perfusion defect prevalence and OS parameters.
Conclusion:
Defects in myocardial perfusion and increase in OS were observed in BD; however, there was no correlation between the two findings in the inactive period. In other words, the prevalence and intensity of myocardial perfusion defects can vary at different OS levels.
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