Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fluorescent chameleon labels for bioconjugation and imaging of proteins, nucleic acids, biogenic amines and surface amino groups. a review.

Methods and applications in fluorescence·2021
Same author

Optical Sensing and Imaging of pH Values: Spectroscopies, Materials, and Applications.

Chemical reviews·2020
Same author

Electrochemical sensors and biosensors using laser-derived graphene: A comprehensive review.

Biosensors & bioelectronics·2020
Same author

Fiber-Optic Chemical Sensors and Biosensors (2015-2019).

Analytical chemistry·2019
Same author

A MXene-Based Wearable Biosensor System for High-Performance In Vitro Perspiration Analysis.

Small (Weinheim an der Bergstrasse, Germany)·2019
Same author

Mn<sup>II</sup>-Doped Cesium Lead Chloride Perovskite Nanocrystals: Demonstration of Oxygen Sensing Capability Based on Luminescent Dopants and Host-Dopant Energy Transfer.

ACS applied materials & interfaces·2018

Related Experiment Video

Updated: Jun 12, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Multiple fluorescent chemical sensing and imaging.

Matthias I J Stich1, Lorenz H Fischer, Otto S Wolfbeis

  • 1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, D-93040 Regensburg, Germany.

Chemical Society Reviews
|June 24, 2010
PubMed
Summary
This summary is machine-generated.

Optical sensors offer unique multi-analyte detection for complex samples. This review covers spectroscopic principles, materials, and applications of dual and triple optical sensors for continuous monitoring.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Related Experiment Videos

Last Updated: Jun 12, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Optical sensors enable simultaneous detection of multiple (bio)chemical species.
  • These sensors utilize probes with spectrally or temporally resolvable signals.
  • Applications include continuous monitoring in complex matrices like blood and industrial fluids.

Purpose of the Study:

  • To critically review the state-of-the-art in optical sensing technologies.
  • To discuss spectroscopic principles and materials used in multi-analyte optical sensors.
  • To highlight examples and future directions for dual and triple sensor systems.

Main Methods:

  • Review of spectroscopic principles for optical sensing.
  • Analysis of indicator probes and polymer materials for sensor development.
  • Compilation and discussion of existing dual and triple optical sensor examples.

Main Results:

  • Optical sensors provide non-invasive, non-toxic, and online detection capabilities.
  • Successful implementation of dual and triple sensors demonstrated across various fields.
  • Advancements in probe and material science are key to enhanced sensor performance.

Conclusions:

  • Optical sensors are highly valuable for multi-analyte detection in diverse applications.
  • Continued research in materials and spectroscopy will drive future innovations in optical sensing.
  • The review provides a comprehensive overview and future outlook for multi-analyte optical sensor technology.