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Updated: Jun 12, 2026

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography
Published on: November 20, 2021
Cryo-scanning electron microscopy and light microscopy for the study of fungi interactions
1Fondo de Diversidad Fúngica de la Comunidad Valenciana, Unidad Docente de Ecología y Botánica, Departamento de Ecosistemas Agroforestales, Escuela Técnica Superior del Medio Rural y Enología, Universidad Politécnica de Valencia, Avda, Blasco Ibañez, 21. 46010-Valencia, España. frasemfe@yahoo.es.
Abstract:
The application of the cryo-scanning electron microscopy and light microscopy for the study of the interactions at different environmental conditions between Penicillium oxalicum and Fusarium verticillioides is described. A dual microculture was developed for the light microscopy analysis of the interaction. The microscope and macroscopic examinations were compared. Analysis of Petri plates revealed that F. verticillioides was a competitor for space and nutrients while P. oxalicum was a mycoparasite under the microscopic observations.
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