A simple method for cell sheet fabrication using mica surfaces grafted with peptide detergent A(6)K

Cell sheet technology is a very important strategy for scaffold-free tissue engineering. In order to fabricate cell sheets by a simple method, peptide detergent A(6)K was grafted on mica surfaces by dropping its aqueous solution at different concentrations on the surface. As revealed by surface topographical observation and water contact angle measurement, the most hydrophobic surface was obtained using peptide solution at the concentration of 0.2 mg · mL(-1). The peptide-grafted mica surface was used to culture mouse preosteoblast cell MC3T3-E1. After the cells reached confluence and the peptide was biodegraded, an intact cell sheet was peeled from the mica. This simple method does not need any non-biological reagents or complicated procedures, and may have great potential in tissue engineering based on cell sheet technology.