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Related Experiment Video

Updated: Jun 12, 2026

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Published on: March 1, 2012

Brome mosaic virus, good for an RNA virologist's basic needs.

C C Kao¹, K Sivakumaran

¹Department of Biology, Indiana University, Bloomington, IN 47405, USA.

Molecular Plant Pathology

|June 25, 2010

Summary

This study details the structure and function of the Brome mosaic virus (BMV), a plant pathogen. It outlines BMV

Area of Science:

Virology
Plant Pathology
Molecular Biology

Background:

Brome mosaic virus (BMV) is a type member of the Bromovirus genus within the Bromoviridae family.
It belongs to the alphavirus-like supergroup of positive-sense, single-stranded RNA viruses.

Purpose of the Study:

To describe the taxonomic relationships, physical properties, genome organization, and protein functions of BMV.
To identify host range and associated symptoms in monocot and dicot plants.

Main Methods:

Characterization of virion structure (nonenveloped icosahedral, 26 nm diameter).
Analysis of the three-segmented positive-sense RNA genome (RNA1, RNA2, RNA3) and subgenomic RNA4.
Identification of viral proteins and their putative functions (RNA helicase, RNA-dependent RNA polymerase, movement protein, capsid protein).

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Main Results:

BMV virions are composed of 180 coat protein subunits with 22% nucleic acid and 78% protein.
RNA1 encodes a protein with capping and RNA helicase activities; RNA2 encodes an RNA-dependent RNA polymerase.
RNA3 codes for a cell-to-cell movement protein and the capsid protein, translated from RNA4.

Conclusions:

BMV infects monocots (Poaceae family) causing brown streaks and dicots (e.g., Nicotiana benthamiana, Chenopodium species) with varying symptoms.
Understanding BMV's molecular biology and host interactions is crucial for managing plant diseases.