Related Experiment Video
Updated: Jun 12, 2026
Merkel Cell Polyomavirus Infection and Detection
Published on: February 7, 2019
Concentration of acrylamide in a polyacrylamide gel affects VP4 gene coding assignment of group A equine rotavirus
LaShanda M Long-Croal1, Xiaobo Wen, Eileen N Ostlund
1Rotavirus Vaccine Development Section, Laboratory of Infectious Diseases, NIAID, National Institutes of Health, Bethesda, MD 20892, USA.
Background:
It is universally acknowledged that genome segment 4 of group A rotavirus, the major etiologic agent of severe diarrhea in infants and neonatal farm animals, encodes outer capsid neutralization and protective antigen VP4.
Results:
To determine which genome segment of three group A equine rotavirus strains (H-2, FI-14 and FI-23) with P[12] specificity encodes the VP4, we analyzed dsRNAs of strains H-2, FI-14 and FI-23 as well as their reassortants by polyacrylamide gel electrophoresis (PAGE) at varying concentrations of acrylamide. The relative position of the VP4 gene of the three equine P[12] strains varied (either genome segment 3 or 4) depending upon the concentration of acrylamide. The VP4 gene bearing P[3], P[4], P[6], P[7], P[8] or P[18] specificity did not exhibit this phenomenon when the PAGE running conditions were varied.
Conclusions:
The concentration of acrylamide in a PAGE gel affected VP4 gene coding assignment of equine rotavirus strains bearing P[12] specificity.
