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Related Experiment Video

Updated: Jun 12, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

Novel RBPJ transcripts identified in human amniotic fluid cells.

Anna Jezierski¹, Dao Ly, Brandon Smith

¹Neurogenesis and Brain Repair, Neurobiology Program, Institute for Biological Sciences, National Research Council Canada, Building M-54, 1200 Montreal Road, Ottawa, Canada.

Stem Cell Reviews and Reports

|June 25, 2010

Summary

Researchers discovered five new RBPJ transcript variants in human amniotic fluid cells, expanding the known forms of this key transcription factor involved in NOTCH signaling and gene regulation.

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Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

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Published on: November 27, 2017

Area of Science:

Molecular Biology
Developmental Biology
Stem Cell Biology

Background:

The NOTCH signaling pathway is crucial for stem cell maintenance, cell-fate determination, and differentiation during development.
The NOTCH intracellular domain (NICD) interacts with the RBPJ transcription factor to regulate gene expression.
Four human RBPJ isoforms are currently known, differing in their 5'coding exons.

Purpose of the Study:

To identify novel RBPJ transcript variants in human amniotic fluid cells.
To compare the expression patterns of RBPJ transcripts in different cell types.
To investigate the functional potential of newly identified RBPJ variants.

Main Methods:

Utilized an improved protocol for identifying RBPJ transcript variants.
Employed human amniotic fluid cells, known for stem cell characteristics.
Used human embryonal carcinoma NTera2/D1 cells and derived neurons/astrocytes for comparative analysis.

Main Results:

Identified four known and five novel RBPJ transcript variants in human amniotic fluid cells.
Observed that novel splice variants possess in-frame open reading frames, suggesting potential for similar protein functions.
Confirmed the presence of a putative nuclear localization signal (NLS) in all identified RBPJ transcripts.

Conclusions:

The discovery of novel RBPJ variants expands our understanding of RBPJ diversity.
These findings suggest a broader regulatory role for RBPJ in gene expression, particularly in stem and progenitor cells.
The conserved NLS in all variants highlights its importance in RBPJ-mediated transcriptional regulation.