Related Experiment Video
Updated: Jun 12, 2026
Applications of Liquid-Chromatography Tandem Mass Spectrometry in Natural Products Research: Tropane Alkaloids as a Case Study
Published on: March 8, 2024
[Determination of tractylodinol in different populations of Atractylodes lancea]
Chengwu Fang1, Min Fan, Shoujin Liu
1Anhui University of Traditional Chinese Medicine, Anhui Key Laboratory of Modernized Chinese Material, Hefei 230031, China. cwfang1961@sina.com
Objective:
To establish an RP-HPLC method for determination of atractylodinol in Ateractylodes lancea and compare the contents of atractylodinol in the herbs of different origins.
Method:
The samples were separated on an Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (49:51). Flow rate was 1.0 mL x min(-1). The detection wave length was set at 337 nm. Column temperature was 30 degrees C.
Result:
The linear range of atractylodinol was 9.12 x 10(-2) -9.12 mg x L(-1) (r = 0.999 9), the average recovery was 97.15%, RSD was 1.5% (n = 5). The contents of atractylodinol were in the range of 0.268-1.213 mg x g(-1) in the samples from different orgins. The contents of atractylodinol in samples growing in Dabieshan mountain were higher than those in Jiangsu province (P < 0.001).
Conclusion:
The established method for determination of atractylodinol is accurate and reliable, which can be used to evaluate the quality of A. lancea, the contents of atractylodinol in the sample was related with its morphological characteristic and geographic orgin.
