Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or basic...
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Janus Synapses as Modular Neurointerfaces.

ACS applied materials & interfaces·2026
Same author

Asymmetric Mass Transport in Polybromide Ionic Liquids and Its Impact on Dual-Plating Zinc Bromine Batteries.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Engineering Cyanoarene Photocatalysts to Resolve Deactivation Challenges in Organocatalyzed ATRP.

Angewandte Chemie (International ed. in English)·2025
Same author

Singlicate bead-based immunoassay for biomarker quantitation in sub-microliter volume sample.

Biosensors & bioelectronics·2025
Same author

Redirecting electron flows in glutamate oxidases by selective anchoring of osmium complexes.

Chemical science·2025
Same author

Distinct Exocytosis Behavior at Synaptic Membrane Protein-Protein Coupled Cell-Electrode Interfaces Revealed by Single-Cell Amperometry.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

High yield sample preconcentration using a highly ion-conductive charge-selective polymer.

Honggu Chun1, Taek Dong Chung, J Michael Ramsey

  • 1Department of Chemistry, University of North Carolina at Chapel Hill, Chapman Hall, CB#3216, Chapel Hill, North Carolina 27599, USA.

Analytical Chemistry
|June 26, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces a novel microfluidic system using poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid) for efficient sample preconcentration. The system effectively concentrates anions, showing promise for proteomic applications in microfluidic devices.

More Related Videos

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
10:27

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

Related Experiment Videos

Last Updated: Jun 12, 2026

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
08:06

Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone

Published on: February 23, 2017

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides
10:27

Preparation of Highly Porous Coordination Polymer Coatings on Macroporous Polymer Monoliths for Enhanced Enrichment of Phosphopeptides

Published on: July 14, 2015

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes
05:26

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Published on: April 29, 2020

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biotechnology

Background:

  • Microfluidic devices offer advantages for sample analysis but often require preconcentration steps.
  • Existing preconcentration methods can be limited by efficiency, robustness, or compatibility with various buffer conditions.

Purpose of the Study:

  • To develop and analyze a microfluidic sample preconcentration system utilizing a novel charge-selective polymer.
  • To investigate the preconcentration mechanism based on concentration polarization.
  • To assess the system's performance with different analytes and buffer compositions.

Main Methods:

  • Development of a robust, ion-conductive polymer, poly-AMPS (2-acrylamido-2-methyl-1-propanesulfonic acid).
  • Integration of the polymer into a microfluidic device between two channels.
  • Utilizing concentration polarization at polymer-buffer interfaces for ion separation.
  • Testing preconcentration efficiency with fluorescein and TRITC-tagged albumin.

Main Results:

  • The poly-AMPS polymer demonstrated stability across a wide range of buffer compositions.
  • Efficient anion preconcentration was achieved through cation extraction by the negatively charged polymer.
  • Linear preconcentration was observed up to 3 mM for fluorescein and 500 microM for albumin.
  • The system showed potential for concentrating proteins on microfluidic platforms.

Conclusions:

  • A robust and versatile microfluidic preconcentration system based on poly-AMPS was successfully developed.
  • The system leverages concentration polarization for efficient anion enrichment.
  • This technology holds significant potential for enhancing sensitivity in microfluidic-based proteomic analyses.