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Related Experiment Video

Updated: Jun 12, 2026

Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
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Nanoglycan complex formulation extends VEGF retention time in the lung.

E Hunter Lauten1, Jarod VerBerkmoes, Justin Choi

  • 1Harvard School of Engineering and Applied Sciences, 29 Oxford Street, Cambridge, Massachussets 02138, USA.

Biomacromolecules
|June 26, 2010
PubMed
Summary

We developed a nanoglycan complex to prolong the lung retention of vascular endothelial growth factor (VEGF) for stem cell therapies. This formulation significantly extends growth factor presence in the lungs, enhancing potential therapeutic effects.

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Area of Science:

  • Biomaterials Science
  • Pulmonary Drug Delivery
  • Regenerative Medicine

Background:

  • Prolonging the lung retention of aerosol-delivered growth factors is crucial for effective stem cell homing and activation.
  • Current delivery methods often result in rapid clearance, limiting therapeutic potential.

Purpose of the Study:

  • To develop and characterize a novel formulation for extended aerosolized delivery of vascular endothelial growth factor (VEGF).
  • To evaluate the in vitro and in vivo performance of VEGF complexed with dextran sulfate (DS) and chitosan (CS) polyelectrolytes.

Main Methods:

  • Formulation of VEGF with dextran sulfate (DS) and chitosan (CS) polyelectrolytes into nanoparticle complexes.
  • Characterization of nanoparticle size, zeta potential, and in vitro VEGF release kinetics.

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  • Assessment of VEGF lung retention in a rat model following aerosol delivery.

    • Main Results:

    • Optimal VEGF incorporation was achieved at a VEGF/DS/CS ratio of 0.12:1:0.33, forming nanoparticles (612+/-79 nm).
    • In vitro studies showed biphasic VEGF release.
    • In rat lungs, aerosolized VEGF in the complex exhibited an 8-fold slower clearance rate compared to free VEGF.

    Conclusions:

    • Complexation with DS and CS significantly extends the lung retention of aerosol-delivered VEGF.
    • The extended retention is attributed to equilibrium binding with glycosaminoglycans.
    • This nanoglycan complex approach shows promise for enhancing lung-based stem cell therapies with other growth factors.