Related Experiment Video
Updated: Jun 12, 2026
Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
Published on: January 10, 2025
Platelet quality after washing: the effect of storage time before washing
Nahreen Tynngård1, Marie Trinks, Gösta Berlin
1Department of Clinical Immunology & Transfusion Medicine, Linköping University Hospital, Sweden.
Insights
Washing platelet concentrates (PCs) on Day 1 or Day 7 of storage similarly affects platelet quality, but washing on Day 7 results in a greater loss of platelets.
Area of Science:
- Transfusion Medicine
- Hematology
- Platelet Biology
Background:
- Washed platelet concentrates (PCs) are used for patients with anaphylactic transfusion reactions.
- Plasma is substituted with platelet additive solution in washed PCs.
Purpose of the Study:
- To compare the in vitro quality of PCs washed on Day 1 versus Day 7 of storage.
- To evaluate the impact of washing timing on platelet surface markers and coagulation properties.
Main Methods:
- Platelets were prepared using the buffy coat procedure and pooled.
- Identical pairs of PCs were washed with T-Sol on Day 1 or Day 7.
- Analyzed swirling, blood gases, metabolic variables, CD62P expression, and coagulation using free oscillation rheometry (FOR).
Main Results:
- pH remained acceptable in all PCs.
- Washing on both Day 1 and Day 7 increased CD62P surface expression.
- Clotting time and clot retraction were unaffected by washing timing.
- A greater decrease in platelet count occurred when washing on Day 7 compared to Day 1.
Conclusions:
- Washing platelet concentrates on Day 1 or Day 7 impacts them similarly regarding quality parameters.
- A significantly larger loss of platelets is observed when washing occurs on Day 7 of storage.
Background:
Patients who have experienced anaphylactic transfusion reactions receive washed platelet (PLT) concentrates (PCs) where the plasma has been substituted with a PLT additive solution. This study compares the in vitro quality of PCs washed at the beginning of the storage period (Day 1) to PCs washed at the end of storage (Day 7).
Study Design And Methods:
PLTs were prepared by the buffy coat procedure. Two concentrates were pooled and then split to obtain an identical pair of PCs. One of the PCs was washed with T-Sol on Day 1 and the other on Day 7 of storage. Swirling, blood gases, and metabolic variables were analyzed before washing. Analyses of surface expression of CD62P and coagulation by free oscillation rheometry (FOR) were performed before and after washing.
Results:
pH was acceptable in all PCs. Washing on Days 1 and 7 increased the CD62P surface expression. The FOR variables clotting time and clot retraction were not influenced by washing on either day. Washing resulted in a decrease in the number of PLTs and the decrease was larger on Day 7 compared to Day 1.
Conclusions:
PLTs washed on Days 1 and 7 of storage are effected by washing in a similar manner. However, a larger loss of PLTs occurred during washing on Day 7.
More Related Videos
05:49Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
Published on: November 29, 2024
09:13Turbidimetry on Human Washed Platelets: The Effect of the Pannexin1-inhibitor Brilliant Blue FCF on Collagen-induced Aggregation
Published on: April 6, 2017
