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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Related Experiment Video

Updated: Jun 12, 2026

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

Published on: October 17, 2010

Laser scanning microscope with a differential heterodyne optical probe.

S Komatsu, H Suhara, H Ohzu

    Applied Optics
    |June 26, 2010
    PubMed
    Summary

    A novel laser scanning microscope visualizes defects and particles in transparent objects. This system achieves high image contrast, even with obscuring particles, using a Zeeman laser and interference effects.

    Area of Science:

    • Optics and Photonics
    • Materials Science
    • Microscopy

    Background:

    • Observing internal structures in partially transparent materials is challenging.
    • Light scattering particles and defects can significantly impact material properties.
    • Existing microscopy techniques may struggle with image clarity in the presence of obscuring elements.

    Purpose of the Study:

    • To develop a new laser scanning microscope system.
    • To visualize the spatial distribution of light scattering particles or defects.
    • To achieve high image contrast in partially transparent objects.

    Main Methods:

    • Utilized a laser scanning microscope with an optical probe.
    • Employed interference between two laser beams of slightly different frequencies for intensity modulation.

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    Related Experiment Videos

    Last Updated: Jun 12, 2026

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)
    12:56

    Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering (CARS)

    Published on: October 17, 2010

    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
    09:13

    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

    Published on: July 6, 2019

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
    10:28

    Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

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  • Used a Zeeman laser and a polarizing optical system to generate coherent beams.
  • Implemented a computer-controlled scan stage for precise movement.

    • Main Results:

    • Demonstrated high image contrast using the developed microscopy method.
    • Successfully visualized defects and particles in latex spheres, microscale targets, and single crystals (LiNbO3, TGS).
    • Showcased the system's effectiveness even when obscuring particles were present in front of the probe volume.

    Conclusions:

    • The new laser scanning microscope system effectively visualizes internal structures.
    • The interference-based intensity modulation provides high contrast imaging.
    • This technique is valuable for defect and particle analysis in transparent materials.