Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modified in-line Sagnac interferometer with passive demodulation technique for environmental immunity of a fiber-optic current sensor.

Applied optics·2008
Same author

Education and imaging. Gastrointestinal: bleeding gastric diverticulum.

Journal of gastroenterology and hepatology·2008
Same author

Simple high-coherence rapidly tunable external-cavity diode laser.

Optics letters·2007
Same author

BCR-ABL oncogenic transformation of NIH 3T3 fibroblasts requires the IL-3 receptor.

Oncogene·2007
Same author

Prostacyclin protects renal tubular cells from gentamicin-induced apoptosis via a PPARalpha-dependent pathway.

Kidney international·2007
Same author

Serum cardiac troponin response in adolescents playing basketball.

International journal of sports medicine·2007
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Jun 12, 2026

Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Microscopic mapping of subnanometric motion.

H Lin, M Sharnoff

    Applied Optics
    |June 26, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This study calibrates a microscope-based holographic system for cell motion analysis. The system can detect ultrafine displacements as small as 1.1 nanometers, with potential for 0.7 nm detection.

    More Related Videos

    Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
    08:17

    Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

    Published on: August 16, 2021

    Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
    09:45

    Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

    Published on: August 8, 2019

    Related Experiment Videos

    Last Updated: Jun 12, 2026

    Picometer-Precision Atomic Position Tracking through Electron Microscopy
    15:04

    Picometer-Precision Atomic Position Tracking through Electron Microscopy

    Published on: July 3, 2021

    Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy
    08:17

    Probing Structural and Dynamic Properties of Trafficking Subcellular Nanostructures by Spatiotemporal Fluctuation Spectroscopy

    Published on: August 16, 2021

    Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
    09:45

    Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

    Published on: August 8, 2019

    Area of Science:

    • Biophysics
    • Optical Microscopy
    • Nanotechnology

    Background:

    • Accurate measurement of cellular component motion is crucial for understanding cell biology.
    • Existing holographic microscopy techniques face limitations in sensitivity for detecting ultrafine displacements.

    Purpose of the Study:

    • To calibrate the sensitivity of a novel microscope-based holographic system.
    • To establish a method for mapping the motion of living cell components with high precision.

    Main Methods:

    • Utilized air bubbles drifting at known velocities in a glycerine-filled capillary.
    • Employed interferometric capture of ultrafine bubble motion using subtractive superposition of holograms.
    • Reconstructed images from interferograms to visualize displacements.

    Main Results:

    • Achieved detection of displacements as small as 1.1 nanometers.
    • Demonstrated a method for visualizing ultrafine motion where stationary and moving parts of the field of view are distinctly rendered.
    • Identified potential for detecting 0.7 nm displacements with apparatus improvements.

    Conclusions:

    • The calibrated holographic system offers high sensitivity for nanoscale motion detection.
    • The methodology provides a viable approach for studying dynamic processes in living cells.
    • Further optimization can enhance the system's capability for even finer displacement measurements.