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Updated: Jun 12, 2026

An Optimized Single-Molecule Pull-Down Assay for Quantification of Protein Phosphorylation
Published on: June 6, 2022
Colorimetric and fluorescent sensing of pyrophosphate in 100% aqueous solution by a system comprised of rhodamine B
Chuda Raj Lohani1, Joung-Min Kim, So-Young Chung
1Bioorganic Chemistry Lab, Department of Chemistry, Inha University, 253 Younghyun-Dong, Nam-Gu, Inchon-City 402-751, Republic of Korea.
Abstract:
We developed a simple dual signal (color and 'Off-On' fluorescent change) ensemble system based on the complex between a rhodamine derivative 1 and Al(3+) for the detection of pyrophosphate (PPi) in 100% aqueous solutions. The complex between the rhodamine compound and Al(3+) was utilized as a chemosensing ensemble for the first time. The ensemble showed highly sensitive and selective fluorescent and colorimetric response to pyrophosphate among the anions in 100% aqueous solutions and no interference of the potent biological competitors including ATP, ADP, and phosphate for the detection of PPi in 100% aqueous solutions at pH 7.4.
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