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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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ExCYT: A Graphical User Interface for Streamlining Analysis of High-Dimensional Cytometry Data
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Published on: January 16, 2019

Web-based analysis and publication of flow cytometry experiments.

Nikesh Kotecha1, Peter O Krutzik, Jonathan M Irish

  • 1Stanford University School of Medicine, Stanford, California.

Current Protocols in Cytometry
|June 26, 2010
PubMed
Summary

Cytobank is a web-based platform for flow cytometry experiments, enabling data storage, analysis, and sharing. It fosters collaboration and transparent access to experimental data and analysis for researchers globally.

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Area of Science:

  • Biotechnology
  • Bioinformatics
  • Computational Biology

Background:

  • Flow cytometry is a critical technique in biological research for analyzing single cells.
  • Managing and analyzing large flow cytometry datasets presents significant challenges.
  • Existing platforms often lack integrated tools for collaborative analysis and data sharing.

Purpose of the Study:

  • To introduce Cytobank, a web-based application designed for flow cytometry data management.
  • To provide a collaborative platform for researchers to store, analyze, and share flow cytometry experiments.
  • To enhance transparency and reproducibility in flow cytometry data analysis and figure generation.

Main Methods:

  • Cytobank utilizes a web browser interface for user access.
  • It offers a suite of tools for basic and advanced flow cytometry analysis.
  • Data and analysis steps are stored centrally, ensuring accessibility and version control.

Main Results:

  • Cytobank facilitates secure, remote access to flow cytometry experiments from any internet-connected computer.
  • The platform supports collaborative analysis, allowing multiple researchers to work on the same experiment.
  • Figure layouts are transparent, linking directly to underlying data and processing steps.

Conclusions:

  • Cytobank provides a comprehensive solution for flow cytometry experiment management and analysis.
  • The platform promotes collaboration and data sharing within the scientific community.
  • Cytobank enhances the transparency and reproducibility of flow cytometry research.