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Differentiating Taenia eggs found in human stools: does Ziehl-Neelsen staining help?
Juan A Jimenez1, Silvia Rodriguez, Luz M Moyano
1Department of Microbiology, School of Sciences, Universidad Peruana Cayetano Heredia, Lima, Perú.
Objective:
To determine whether Ziehl-Neelsen staining can differentiate Taenia solium from Taenia saginata eggs.
Methods:
Tapeworm proglottids (33 specimens, 23 T. solium and 10 T. saginata) and eggs (31 specimens, 13 T. solium and 18 T. saginata) were stained. Four eggs from each sample were measured and average diameters were recorded.
Results:
Taenia saginata eggs stained entirely magenta in seven of 13 cases. Taenia solium eggs stained entirely blue/purple in 4/18 cases and entirely magenta in one. Eggs of T. saginata were slightly larger and always ovoid, while T. solium eggs were smaller and mostly spheric.
Conclusions:
Ziehl-Neelsen staining can occasionally distinguish fully mature T. solium from T. saginata eggs, but this distinction is neither very sensitive nor completely specific. Differential staining suggests differences in embryophore components between species which become evident with egg maturation. In this small series, egg morphology (shape, maximal diameter) provided appropriate differentiation between T. solium and T. saginata eggs.
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