[Human limbal epithelial cell growth kinetics in vitro]
V Borderie1, P Borderie, E Basli
1U968, plateforme de thérapie cellulaire, centre de recherche, institut de Vision, Inserm, université Pierre-et-Marie-Curie, CHNO des Quinze-Vingt, 28, rue de Charenton, 75571 Paris cedex 12, France. vincent.bordereie@upmc.fr
Journal Francais D'Ophtalmologie
|June 29, 2010
Summary
Limbal epithelial cells from organ-cultured donor corneas show growth potential for transplantation. However, postmortem ischemia significantly impairs cell renewal, emphasizing timely tissue retrieval.
Area of Science:
- Ophthalmology
- Regenerative Medicine
- Cell Biology
Context:
- Donor corneas preserved in organ culture up to 3 weeks are a source for limbal explants.
- Limbal epithelial stem cells are crucial for corneal surface reconstruction.
- Understanding cell kinetics is vital for optimizing cell-based therapies.
Purpose:
- To evaluate the in vitro growth kinetics of limbal epithelial cells derived from organ-cultured donor corneas.
- To determine the viability and proliferative capacity of these cells for potential clinical applications.
Summary:
- Limbal explants from organ-cultured donor corneas (18-24 days preservation) were cultured for 10-18 days.
- Epithelial sheet area was measured morphometrically, and cell proliferation was assessed.
- Cell cycle duration averaged 24 hours, with growth declining relative to postmortem time.
Impact:
- Organ-cultured limbal tissue retains potential for limbal allograft and cultured stem cell transplantation.
- Donor postmortem ischemia negatively affects cell growth, necessitating prompt tissue procurement.
- Findings support the clinical use of limbal tissue from extended organ culture, with caveats on donor age.
